Phage Production in Alternative Media: A Comparative Analysis of Pseudomonas aeruginosa Growth in Animal-Free LB Medium and Chemically Defined Medium
Summary
Biopharmaceutical Biotechnology
Public
Laboratory of Bacteriophages and Phage Therapy, Center for Research and Innovation in Pharmaceutical Clinical Sciences (CRISP), Lausanne University Hospital (CHUV), Dr. Grégory Resch / Cell Production Center (CPC), Service of Pharmacy, Lausanne University Hospital (CHUV), Dr. Jean-François Brunet
Carmen Jungo Rhême
Skills directory
November 2024 - August 2025
Lysogeny Broth (LB) medium is widely used for bacteriophage production; however, for therapeutic applications, costly animal-free LB formulations are required to comply with regulatory standards. This study compares the growth performance of Pseudomonas aeruginosa and the kinetics of phage amplification with an animal-free LB medium and a chemically defined medium.
In 2024, the P. aeruginosa phage production and purification process developed at the CHUV by the teams of Dr. Grégory Resch and Dr. Jean-François Brunet was successfully transferred to the Biofactory Competence Center (BCC). The use of chemically defined medium was evaluated and successfully implemented in 2024 for improved production of phages.
In this study, the growth performance of P. aeruginosa and the kinetics of phage-bacteria interactions are compared for several types of phages in animal-free LB medium and a chemically defined medium.